Gainesville-Hawthorne State Trail is a 30 mile lightly trafficked out and back trail located near Gainesville, Florida that offers the chance to see wildlife and is rated as moderate. The trail is primarily used for walking, trail running, horses, and road biking and is accessible year-round. Horses are also able to use this trail.
I've been riding this trail for years and it never gets old. With every season comes a new trail. Smooth path, pretty frequent scenic stops. A must-ride if you are in the area.
One of the best trails around for road biking. Watch out for heavy pedestrian/rollerblade/stroller traffic the first couple of miles, but after that the traffic fades away and you get to experience many different ecosystems with little traffic (except the occasional cross street) to worry about. Hit up the trail early in the morning and you will likely see turkeys and deer, and maybe even a wild boar. Just don't get too relaxed, as these critters are unpredictable (I came up on a guy who had somersaulted his bike over a wild boar one time).
Really, the Gainesville-Hawthorne trail is one of the true gems in this area, a very scenic ride, and a great way to get some shaded exercise in the brutal Florida summer.